FastQCFastQC Report
Wed 15 Apr 2020
FCHHWFYBBXX_L3_CHKPEI85217070034_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHHWFYBBXX_L3_CHKPEI85217070034_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100736229
Sequences flagged as poor quality0
Sequence length49
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG7944350.7886288854429919No Hit
CACACACACACACACACACACACACACACACACACACACACACACACAC6252230.6206535684396127No Hit
GTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGACGCTGCC1865180.18515483639952415No Hit
GTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGACGCTG1834410.18210032460119188No Hit
CACACACACACACACACACACACCTGTCTCTTATACACATCTGACGCTG1669620.16574176108974656No Hit
CACACACACACACACACACACCTGTCTCTTATACACATCTGACGCTGCC1631880.1619953433039468No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGACGC1605470.1593736450071007No Hit
CACACACACACACACACACACACACCTGTCTCTTATACACATCTGACGC1516110.15050295360966906No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAG1377530.13674623456472645No Hit
GTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGACGCTGCCGA1367100.1357108573123181No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTGAC1358390.13484622300086296No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC1334310.13245582182751747No Hit
CACACACACACACACACACACACACACCTGTCTCTTATACACATCTGAC1321840.13121793550560643No Hit
CACACACACACACACACACCTGTCTCTTATACACATCTGACGCTGCCGA1178160.11695494378690709No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGCTGTCTCTTATACACATCTG1163140.11546392112811767No Hit
CACACACACACACACACACACACACACACCTGTCTCTTATACACATCTG1114090.11059476923639855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGA541850.022.28421843
GCCGACG209800.019.191343
TGCCGAC213750.018.93653542
GCTGCCG674100.018.1189642
ACGCTGC1013250.017.68256842
CGCTGCC1041500.017.34185243
GGGTATA75200.012.682521
TGACGCT1468950.012.52630742
GACGCTG1486150.012.33983543
CTATACT353600.011.7673284
TAACAGT494100.011.4660884
GTACTAA141950.011.4279981
TATACTG445000.011.1914055
GTATTAT216900.011.1332331
GTCCTAC87800.010.9864791
CCTATAC306550.010.9302033
GTATTAA205250.010.7891461
GTATAAA290700.010.5689351
TTATACT189400.010.5532874
GGCCTAT323200.010.4830471
Produced by FastQC (version 0.11.5)